[Bioperl-l] Missing parameters in Bio::Tools::Run::Primer3
Brian Osborne
osborne1 at optonline.net
Fri Dec 9 11:36:23 EST 2005
Rasmus,
Thanks for pointing this out. Please record this in Bugzilla
(http://bugzilla.bioperl.org) as an "Enhancement", this way your suggestion
won't get lost.
Brian O.
On 12/9/05 4:36 AM, "Rasmus Ory Nielsen" <ron at ron.dk> wrote:
> Hi list
>
> I wish to use the Bio::Tools::Run::Primer3 module (branch 1-5-1). I
> found that the PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS parameter is missing
> from the module. This is the warning I get:
>
> MSG: Parameter PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS is not a valid
> Primer3 parameter
>
> I then searched the module for more missing parameters. Below is a list
> of what I found.
>
> PRIMER_DEFAULT_PRODUCT
> PRIMER_DEFAULT_SIZE
> PRIMER_INSIDE_PENALTY
> PRIMER_INTERNAL_OLIGO_MAX_TEMPLATE_MISHYB
> PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS
> PRIMER_MAX_TEMPLATE_MISPRIMING
> PRIMER_OUTSIDE_PENALTY
> PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING
> PRIMER_PAIR_WT_TEMPLATE_MISPRIMING
> PRIMER_WT_TEMPLATE_MISPRIMING
>
> Best regards
> Rasmus Ory Nielsen
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