[Bioperl-l] Missing parameters in Bio::Tools::Run::Primer3

Rasmus Ory Nielsen ron at ron.dk
Fri Dec 9 04:36:47 EST 2005


Hi list

I wish to use the Bio::Tools::Run::Primer3 module (branch 1-5-1). I
found that the PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS parameter is missing
from the module. This is the warning I get:

MSG: Parameter PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS is not a valid
Primer3 parameter

I then searched the module for more missing parameters. Below is a list
of what I found.

PRIMER_DEFAULT_PRODUCT
PRIMER_DEFAULT_SIZE
PRIMER_INSIDE_PENALTY
PRIMER_INTERNAL_OLIGO_MAX_TEMPLATE_MISHYB
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS
PRIMER_MAX_TEMPLATE_MISPRIMING
PRIMER_OUTSIDE_PENALTY
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING
PRIMER_WT_TEMPLATE_MISPRIMING

Best regards
Rasmus Ory Nielsen


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