[Bioperl-l] Re: [Gmod-schema] load_gff3 handles ##sequence-region
Scott Cain
cain at cshl.org
Mon Nov 10 12:52:26 EST 2003
Allen,
Thanks for doing this, but it seems I hadn't thought this all the way
through--specifying a name, a start and an end is not sufficent,
therefore, the '##sequence-region' line is not going to work in it's
current form. The problem lies in assigning a SO type to the region.
Is it a chromosome, contig, assembly, band, arm, etc?
So I think we will have to go back to requiring that the reference
sequence be a full GFF line that occurs in the file before any features
referring to it.
Thanks,
Scott
On Fri, 2003-11-07 at 20:51, Allen Day wrote:
> >From chado's load_gff3.pl POD:
>
> "
> Also, in order for the load to be successful, the reference sequences (eg,
> chromosomes or contigs) must be defined in the GFF file before any
> features on them are listed. This can be done either by the
> reference-sequence meta data specification, which would be lines that look
> like this:
>
> ##sequence-region chr1 1 246127941 ----except that this isn't supported
> yet--can I get Bio::Tools::GFF to give me this info?
> "
>
> this is now fixed on bioperl-live HEAD Bio::Tools::GFF. I've added
> parsing capability for the ##sequence region header tag, and stubbed out
> handling of other header tags from the GFF3 spec such as "##attribute
> ontology".
>
> segments are created as Bio::LocatableSeq objects and available via
> Bio::Tools::GFF::next_segment().
>
> -allen
>
>
>
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--
------------------------------------------------------------------------
Scott Cain, Ph. D. cain at cshl.org
GMOD Coordinator (http://www.gmod.org/) 216-392-3087
Cold Spring Harbor Laboratory
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