[Bioperl-l] Restriction Enzymes

[simon andrews (BI)]

-----Original Message-----
From: Roger Hall [mailto:roger@iosea.com]
Sent: Wednesday, August 22, 2001 12:03 PM
To: Bioperl
Subject: [Bioperl-l] Restriction Enzymes

> Please help me know that the data was correct (and the fix that 
> followed): are there restriction enzymes that cleave at the beginning 
> (or end) of the given sequence?

Apparently so.  A quick search through the latest release of rebase shows
about 100 enzymes with cleavage points at the end of their recognition
sequence.  A few examples are shown below ....

	 BscFI     0 'GATC_         4 
	 Bsp143I   0 'GATC_         4 
	BssKI      0 'CCnGG_        5 
	 BstENII   0 'GATC_         4 
	 DpnII     0 'GATC_         4 
	 EcoHI     0 'CCsGG_        5 
	 EcoRII    0 'CCwGG_        5 
	BciVI     12 GTATCCnnnnn_n'  -1 
	 BfiI     11 ACTGGGnnnn_n'  -1 
	 BfuI     12 GTATCCnnnnn_n'  -1 
	BmrI      11 ACTGGGnnnn_n'  -1 
	BplI      24 GAGnnnnnCTCnnnnnnnn_nnnnn'  -5 
	BpmI      22 CTGGAGnnnnnnnnnnnnnn_nn'  -2 
	 BsaMI     7 GAATG_Cn'     -2 
	BsrI       6 ACTG_Gn'      -2 
	BsrDI      8 GCAATG_nn'    -2 
	 BsrSI     6 ACTG_Gn'      -2 
	BstF5I     7 GGATG_nn'     -2 
	BtsI       8 GCAGTG_nn'    -2 
	 ChaI      4 _GATC'        -4 
	 Hsp92II   4 _CATG'        -4 
	MboII     13 GAAGAnnnnnnn_n'  -1 
	MlyI      10 GAGTCnnnnn'    0 
	 Mva1269I   7 GAATG_Cn'     -2 
	NlaIII     4 _CATG'        -4 
	 PctI      7 GAATG_Cn'     -2 


Simon.