[EMBOSS] eprimer3 does not interface properly with primer3_core

Horea Christian h.chr at mail.ru
Sat Mar 28 22:28:19 UTC 2015

I was able to reproduce this issue with primer3-2.3.6 as well.

On Sa, 2015-03-28 at 22:42 +0100, Horea Christian wrote:

> As seen in this discussion [https://www.biostars.org/p/102268/]
> primer3 (primer3_core) needs the SEQUENCE_TEMPLATE to be set in a
> config file. Apparently eprimer does not do this.
> This discussion
> [https://www.mail-archive.com/emboss@lists.open-bio.org/msg01980.html]
> is referenced in the above thread to support the claim that primer32
> should be used instead, but it actually states that the proper
> function to use for primer3-2.3.* is primer3.
> My versions:
> emboss-6.6.0
> primer3-2.3.5
> Could you help me out? 
> _______________________________________________
> EMBOSS mailing list
> EMBOSS at mailman.open-bio.org
> http://mailman.open-bio.org/mailman/listinfo/emboss

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