[EMBOSS] eprimer3 does not interface properly with primer3_core

Horea Christian h.chr at mail.ru
Sat Mar 28 21:42:26 UTC 2015


As seen in this discussion [https://www.biostars.org/p/102268/] primer3
(primer3_core) needs the SEQUENCE_TEMPLATE to be set in a config file.
Apparently eprimer does not do this.

This discussion
[https://www.mail-archive.com/emboss@lists.open-bio.org/msg01980.html]
is referenced in the above thread to support the claim that primer32
should be used instead, but it actually states that the proper function
to use for primer3-2.3.* is primer3.

My versions:
emboss-6.6.0
primer3-2.3.5

Could you help me out?
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