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I was able to reproduce this issue with primer3-2.3.6 as well.<BR>
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On Sa, 2015-03-28 at 22:42 +0100, Horea Christian wrote:<BR>
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As seen in this discussion [<A HREF="https://www.biostars.org/p/102268/">https://www.biostars.org/p/102268/</A>] primer3 (primer3_core) needs the SEQUENCE_TEMPLATE to be set in a config file. Apparently eprimer does not do this.<BR>
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This discussion [<A HREF="https://www.mail-archive.com/emboss@lists.open-bio.org/msg01980.html">https://www.mail-archive.com/emboss@lists.open-bio.org/msg01980.html</A>] is referenced in the above thread to support the claim that primer32 should be used instead, but it actually states that the proper function to use for primer3-2.3.* is primer3.<BR>
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My versions:<BR>
emboss-6.6.0<BR>
primer3-2.3.5<BR>
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Could you help me out?
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