[EMBOSS] problem with eprimer32

[David Bauer]

Hi Guy,

in the current EMBOSS there are 2 wrappers for eprimer:
####
eprimer3     Pick PCR primers and hybridization oligos
eprimer32    Pick PCR primers and hybridization oligos
####

eprimer3 is for primer3  version 2.3.x (works for me with 2.3.6) 
and
eprimer32 is for the older version 2.2.3

So if you want to use the new primer3 version (2.3.5), the symbolic link is not needed.
Just put primer3_core in the path and use eprimer3.

Kind regards,
David.

-----Ursprüngliche Nachricht-----
Von: emboss-bounces at lists.open-bio.org [mailto:emboss-bounces at lists.open-bio.org] Im Auftrag von Guy Bottu
Gesendet: 13 February 2014 18:17
An: emboss at lists.open-bio.org
Betreff: [EMBOSS] problem with eprimer32

Dear all,

I installed on my computer EMBOSS version 6.6.0.0 and tried to make 
eprimer32 work. I installed the last version of Primer3 (version 2.3.5) 
and I put a logical link in the bin directory of EMBOSS (primer32_core 
-> .../primer3_core). When I try to run it, I get :

Pick PCR primers and hybridization oligos
Whitehead primer3_core program output file [emboss_001.eprimer32]:
Error: thermodynamic approach chosen, but path to thermodynamic 
parameters not specified

What could be missing ? The EMBOSS eprimer32 manual does not say 
anything beyond the need to have eprimer3_core in the path.

Regards,
Guy Bottu


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