[EMBOSS] meaning of "(Reversed)" in diffseq?

Andres Pinzon andrespinzon at gmail.com
Wed Mar 25 21:04:23 UTC 2009

I extracted this from the EMBOSS documentation, perhaps it'll help you
(specially the las paragraph):
Each report consists of 4 or more lines.

   - The first line has the name of the first sequence followed by the start
   and end positions of the mismatched region in that sequence, followed by the
   length of the mismatched region. If the mismatched region is of zero length
   in this sequence, then only the position of the last matching base before
   the mismatch is given.
   - If a feature of the first sequence overlaps with this mismatch region,
   then one or more lines starting with 'Feature:' comes next with the type,
   position and tag field of the feature.
   - Next is a line starting "Sequence:" giving the sequence of the mismatch
   in the first sequence.

 This is followed by the equivalent information for the second sequence, but
in the reverse order, namely 'Sequence:' line, 'Feature:' lines and line
giving the position of the mismatch in the second sequence.[...]"

On Wed, Mar 25, 2009 at 4:47 PM, Francoeur, Joseph A. <jfrancoe at mitre.org>wrote:

> Hello,
> I ran  diffseq on 2  FASTA-formatted DNA sequence files on my local
> installation of EMBOSS, and I have entries in the .diffseq output file
> labeled as "(Reversed)".  Looking at both sequences in an editor, neither of
> these sequence segments is reversed (the "Sequence" string in the diffseq
> file matches the original FASTA file).  Does anyone know what is meant by
> this?  I'm analyzing the EMBOSS source code, and it looks as though it's
> related to a strand feature.  That's puzzling me, because my local
> installation has no databases installed, and it seems that this kind of
> information wouldn't be embedded in the FASTA file.  How does diffseq
> determine this strand information, and how should I interpret it?
> Thanks,
> Joe
> ---
> Joseph A. Francoeur
> Senior Software Systems Engineer
> The MITRE Corporation
> 202 Burlington Road  MS K228
> Bedford, MA  01730-1420
> e-mail: jfrancoe at mitre.org<mailto:jfrancoe at mitre.org>
> _______________________________________________
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> EMBOSS at lists.open-bio.org
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Andrés Pinzón
Bioinformatics Center, Colombia EMBnet node
Tel +57 3165000 ext 16961 Fax +571 3165415
Micology and Phytopathology Laboratory - Los Andes University.
Tel +571 3394949 ext. 2768

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