[EMBOSS] Some question

[Guy Bottu]

On Thu, Jul 21, 2005 at 12:58:58PM +0100, Peter Rice wrote:
> Kerti Bal?zs G?bor wrote:
> 
> > There is some (elementary) question, because I do not find - maybe I do wrong 
> > - the solution.
> > 
> > - how to backtranslate a cds mRNA fragm. to (c)DNA fragm. ?
> 
> The cDNA will be identical to the mRNA. No backtranslation needed. 
> Backtranslation (as in backtranseq) converts a protein sequence into a 
> nucleotide sequence that will translate to the same protein sequence (using 
> the most frequent codon for each amino acid).
> 
> If you only want to convert U (Uracil) to T (thymine) to convert an RNA 
> sequence to DNA (all EMBOSS programs will accept both as nucleotide input) you 
> can modify the program seqret to specify a nucleotide sequence as input, and 
> generate a DNA sequence as output. An easy way to start writing EMBOSS 
> programs - copy one program and one ACD file and make 4 small edits.

No need to modify seqret, the EMBOSS program biosed can be used to replace 
U by T in a sequence.

	Guy Bottu,
	Belgian EMBnet Node