[EMBOSS] Some question

[Peter Rice]

Kerti Balázs Gábor wrote:

> There is some (elementary) question, because I do not find - maybe I do wrong 
> - the solution.
> 
> - how to backtranslate a cds mRNA fragm. to (c)DNA fragm. ?

The cDNA will be identical to the mRNA. No backtranslation needed. 
Backtranslation (as in backtranseq) converts a protein sequence into a 
nucleotide sequence that will translate to the same protein sequence (using 
the most frequent codon for each amino acid).

If you only want to convert U (Uracil) to T (thymine) to convert an RNA 
sequence to DNA (all EMBOSS programs will accept both as nucleotide input) you 
can modify the program seqret to specify a nucleotide sequence as input, and 
generate a DNA sequence as output. An easy way to start writing EMBOSS 
programs - copy one program and one ACD file and make 4 small edits.

> - how to generate antisense DNA fragm. from a sens.

In EMBOSS, revseq does this. The antisense strand is smilpy the reverse 
compleemnt of the original.

Hope this helps,

Peter Rice