[Bioperl-l] Bio::Tools::SeqStats to count bases
Fields, Christopher J
cjfields at illinois.edu
Mon Feb 4 18:54:39 UTC 2013
Please make sure and read both Roy's and Adam's responses all the way through; Bio::SeqIO is not a sequence object but the front-end for format parsing (e.g. FASTA, etc). Bio::PrimarySeq does not have a '-file' parameter, Bio::SeqIO does.
If SeqStats truly doesn't work with Bio::Seq we can fix that, but according to Adam he has tested using Bio::SeqIO out and it seems to work.
chris
On Feb 4, 2013, at 12:02 PM, Slym <whereverroadgoes at gmail.com>
wrote:
> The thing is, if I use Bio::SeqIO then Bio::Tools::SeqStats produces an
> error (saying that it wants input provided by Bio::PrimarySeq).
> (btw in this line
> $seqobj = Bio::PrimarySeq->new( -file => "sekw2.fasta", - alphabet =>
> 'dna',);
> there's a typo "sekw2" instead of "seq2" but this is correct in my original
> code).
>
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