Yes, BLAST uses fasta files. You (may need to concatenate the individual chromosomes and the you) need to index them with 'makeblastdb' which is also part of the blast+ software package. see: http://www.ncbi.nlm.nih.gov/books/NBK1762/ Hans On 09/22/2011 01:49 PM, Manju Rawat wrote: > It will work on Local Blast or not??????