[Bioperl-l] snp/frameshift identification
Ross KK Leung
ross at cuhk.edu.hk
Sun Sep 18 10:51:05 UTC 2011
Dear Bioperl-users,
Following Fields, Christopher J's advice on sequence extraction, I manage to
proceed to the last stage of non-synonymous SNP identification. Now what I
have in hand is thousands of reliable multiple sequence alignment files,
e.g.
>seq1
ATGACAGACACGACGTTGCCGTAG
>seq2
ATGACAGACACGACGTAGCCGTAG
>seq3
ATGACAGACACGACGTTGCCGTAG
Seq2 has a T->A mutation and that leads to a stop codon generation. I wonder
if Bioperl has handled this kind of SNP or frameshift or non-sense mutations
that lead to change of amino acid in the translated protein product.
Thanks again to the community that helps me a great deal so I can catch up
progress during this Sat/Sun!!
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