[Bioperl-l] use blast to extract similar sequences

Ross KK Leung ross at cuhk.edu.hk
Fri Sep 16 08:51:38 UTC 2011

I wonder whether bioperl has any built-in modules that extracts sequences based on blast results. For example, a short query sequence of length 1000 is to blast against a reference genome of 3M. The homologous sequence of 1000 +/-  20 is extracted. Why is +/- 20 needed? Because we can't guarantee there must have a good match. Frequent blast users may be well aware that then there can be coverage, split-up due to local alignments, etc and that's why I would like to know if anybody has already developed a module to handle this kind of problem. Thanks in advance!


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