[Bioperl-l] Fwd: Doubt - Regarding Stand Alone Blast

[sukeerthi teja Rallapalli]

---------- Forwarded message ----------
From: sukeerthi teja Rallapalli <tejaminnu at gmail.com>
Date: Tue, May 3, 2011 at 7:45 AM
Subject: Re: [Bioperl-l] Doubt - Regarding Stand Alone Blast
To: Dave Messina <David.Messina at sbc.su.se>


Hi David,

I basically have these 2 files, the

Input file        = sequences.txt
Database file  = homologene_result.txt

Isn't "blastall" an older version command ? That is why i was using
makeblastdb

This is what i typed

172-30-8-231:bin sukeerthiteja$ makeblastdb -in homologene_result.txt
-dbtype prot -parse_seqids -out teja1 blastp -query sequences.txt -db teja2

AND i also tried the code using .txt next to *teja2.txt, * but in both cases
i got this error.

Error: (106.18)
NCBI C++ Exception:
    Error: (CArgException::eSynopsis) Too many positional arguments (1), the
offending value: blastp
    Error: (CArgException::eSynopsis) Application's initialization failed

Thanking you
Teja



-- 
Regards

Rallapalli Sukeerthi Teja