[Bioperl-l] about pI prediction

[Frank]

Hi, Thanks all, I wil install them and do some analysis.    I did it via 
net in http://us.expasy.org/tools/pi_tool.html before.  It is not so 
convenient for mass data analysis.

Hi, Brian, do you have any idea about the principle of cacluate pI?  I 
searched some references published in 1980s. I am not sure whether it is 
out of date.

Frank

Brian Osborne wrote:

>Frank,
>
>I've used both Tools/pICalculator and Tools/SeqStats.
>
>Brian O.
>
>-----Original Message-----
>From: bioperl-l-bounces at portal.open-bio.org
>[mailto:bioperl-l-bounces at portal.open-bio.org]On Behalf Of Marc Logghe
>Sent: Tuesday, May 17, 2005 4:09 PM
>To: Frank; bioperl-l at portal.open-bio.org
>Subject: RE: [Bioperl-l] about pI prediction
>
>
>Hi Frank,
>  
>
>>Dose anyone use the  bioperl module to predict pI and 
>>molecular weight 
>>of a protein?   Thanks
>>    
>>
>Don't know of a pure BioPerl module to predict pI. You can use
>Bio::Tools::SeqStats to get the molecular weight, though.
>If you have EMBOSS installed, you can use the Bioperl wrapper to the
>EMBOSS application 'pepstats' for both the pI and mol weight. Have a
>look at the docs for Bio::Factory::EMBOSS.
>However, you have to parse out the corresponding values. The EMBOSS
>docs, including an example output you can find at
>http://emboss.sourceforge.net/apps/pepstats.html
>HTH,
>Marc
>
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