[Bioperl-l] [help] bl2seq using blastp
aplykimo
aplykimo at yahoo.com.tw
Wed May 11 11:23:47 EDT 2005
HI,
I use bl2seq to align two sequence. In file "i.fa" the
first seq is
>1
TSTSCTTTATCAGGATCACCAGGCCCCATCNGGATTCYCMAGAACCCCCAGCCCCAGGGACAG
.
In file "j.fa" Second seq is
>2
MTGGARGACAGATGAGGACCACACCCGCAACCCCCAAGCCAGGACCAGCATCAGTGTCC.
Using command " bl2seq -i i.fa -j j.fa -p blastp -o
bl2seq.out -G 10 -E 1 -W 2 -g F -M BLOSUM62". However,
it output "[NULL_Caption] WARNING: [000.000]
SetUpBlastSearch failed."
I try many different parameters, but it doesn't work
at all. Is there any one know how to solve this
problem?
thx
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