[Bioperl-l] help me with Bio-Tools-PSort-2.0.4

[sasalacolla at libero.it]

Hi Bioperler,  
please rescue me.  
I tried to use a local PSORTb program in $PSORT_ROOT/bin contained in the    
Psortb module: Bio-Tools-PSort-2.0.4 (available at    
http://www.psort.org/downloads/index.html), but I only got this error message:  
  
  
Fatal error:  
------------- EXCEPTION  -------------  
MSG: blastall call crashed: -1 /usr/bin/blastall -p  blastp   
-d  /usr/local/psort/conf/analysis/sclblast/gramneg/sclblast   
-i  /tmp/OsUPf63CDs  -e  1e-09  -o  /tmp/8JSpO7hLfz  -F  F  
  
STACK  
Bio::Tools::Run::StandAloneBlast::_runblast /usr/share/perl5/Bio/Tools/Run/StandAloneBlast.pm:732  
STACK  
Bio::Tools::Run::StandAloneBlast::_generic_local_blast /usr/share/perl5/Bio/Tools/Run/StandAloneBlast.pm:680STACK  
Bio::Tools::Run::StandAloneBlast::blastall /usr/share/perl5/Bio/Tools/Run/StandAloneBlast.pm:536  
STACK  
Bio::Tools::Run::SCLBlast::blast /usr/local/share/perl/5.8.4/Bio/Tools/Run/SCLBlast.pm:134  
STACK  
Bio::Tools::PSort::Module::SCLBlast::run /usr/local/share/perl/5.8.4/Bio/Tools/PSort/Module/SCLBlast.pm:72  
STACK  
Bio::Tools::PSort::Pathway::__ANON__ /usr/local/share/perl/5.8.4/Bio/Tools/PSort/Pathway.pm:194  
STACK  
Bio::Tools::PSort::Pathway::traverse /usr/local/share/perl/5.8.4/Bio/Tools/PSort/Pathway.pm:157  
STACK  
Bio::Tools::PSort::classify /usr/local/share/perl/5.8.4/Bio/Tools/PSort.pm:160  
STACK (eval) /usr/local/bin/psort:318  
STACK toplevel /usr/local/bin/psort:318  
  
--------------------------------------  
  
  
Reading the configuration    
instructions of this module i set the following environmental variables in    
bashrc:    
    
export PSORT_ROOT='/usr/local/psort'    
export PSORT_HMMTOP='/home/sandro/Inst_tools_Rev_Vacc/PSORTb/hmmtop2.1'    
export PSORT_PFTOOLS='/usr/bin'    
export BLASTDIR='/usr/bin'    
    
since blastall is located in my machine in /usr/bin/blastall.   
I tried to solve the problem setting in bashrc:  
export BLASTDATADIR='/conf/analysis/sclblast/gramneg'.   
Anyway nothing changed in the error message, and nothing changes even setting  
absurd addresses for BLASTDATADIR!  
 
Any suggestion? thank you all,guys 



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