[Bioperl-l] Bio::SeqFeature::Primer Calculating the Primer TM

[Rob Edwards]

Barry,

Thanks for this code. I really appreciate having an alternate method 
for calculating this. When I wrote the module I just couldn't figure 
out why the numbers wouldn't match. It seems Tm calculations are not as 
straightforward as they should (?) be. I always use 60C for my PCR 
annealing step and it works 99% of the time :-)

I added your code to Bio::SeqFeature::Primer in CVS and updated 
t/Primer.pm too so that it passes.

I left the original calculation in the module, but renamed it 
Tm_estimate so that it can be there for comparative purposes.

Rob