[Bioperl-l] Bio::tools::primer3 and bio::seqfeature::primer

Rob Edwards rob at salmonella.org
Fri Dec 10 11:33:34 EST 2004

This is already available:

Bio::Tools::Primer3->next_primer() returns a Bio::Seq::PrimedSeq object.

Take a look at the Bio::Seq::PrimedSeq docs to see how to write out a 
sequence with it's primers attached.


On Dec 10, 2004, at 5:02 AM, Sean Davis wrote:

> I am running primer3 using bio::tools::run::primer3.  I would like to 
> add the resulting primers to my original sequence as sequence 
> features.  I can extract all the tags and build my own sequence 
> feature (bio::seqfeature::primer), but the perldoc for 
> bio::seqfeature::primer implies that it is designed to be integrated 
> with bio::tools::primer3.  I just want to make sure that there isn't 
> an inheritance that I don't see before constructing my own 
> Bio::Seqfeature::Primer from the Bio::Tools::Primer3 objects.
> Thanks,
> Sean
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