[Bioperl-l] All mouse mRNAs
Sean Davis
sdavis2 at mail.nih.gov
Thu Apr 29 13:42:00 EDT 2004
Hilmar and others,
Thanks to everyone who replied. I was interested in essentially dbEST
entries for mouse which is available as a single file via FTP from ncbi in
the dbest directories (at least the FASTA). It looks like the file is
compiled and updated on a relatively regular basis and contains all ESTs for
mouse contained in genBank. There is an analogous file for human, if anyone
is in need of such a thing.
Sean
On 4/29/04 12:16 PM, "Hilmar Lapp" <hlapp at gmx.net> wrote:
> Do you want all mouse mRNA sequences that were ever submitted to
> GenBank, or do you want mRNAs for all mouse genes?
>
> In the latter case, RefSeq nicely partitions the release by species
> (see the species-specific cumulative updates).
>
> Genbank is partitioned too, so you don't have to parse everything.
>
> At any rate, NCBI will not be thrilled by people downloading 100s of
> thousands of sequences through their query interfaces ...
>
> -hilmar
>
> On Thursday, April 29, 2004, at 07:40 AM, Sean Davis wrote:
>
>> All,
>>
>> Sorry to bother with a general question, but I am interested in
>> getting all
>> mouse mRNA sequences from genbank. I only need fasta, but there are
>> approximately 300k of these. Is this even worth attempting via bioperl
>> (Bio::DB::Query::Genbank) or is there another way to do this (besides
>> locally installing genBank, if I can help it).
>>
>> Thanks,
>> Sean
>>
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