[Bioperl-l] a space in Feature key in the Feature Table.
hyupaik at indiana.edu
hyupaik at indiana.edu
Fri Oct 17 14:18:37 EDT 2003
Hello list,
It is impossible to have a space in Feature key, Right?
I downlaoded some data from embl cpgisle. They look like below.
---------------------------------------------------------------------------
ID GAPDHG
AC J04038;
LE 5378
DE Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, complete
cds.
DE 7/95
EX Gene expression widespread
FT CpG island 871..1673
FT /size=803
FT /%(C+G)=69.12
FT /Obs/Exp CpG=0.82
FT CpG island 1683..2063
FT /size=381
FT /%(C+G)=67.19
FT /Obs/Exp CpG=0.77
XX
FT /CAAT-box.1="884"
FT /CAAT-box.2_complement="2156"
FT /GC-box="1064"
FT /E2F_CS.1="1785"
FT /SpI="158,1198,1244,1290,1310,1314"
FT /SpI_complement="174,584,1519,1668,1736,2271"
FT /SpI_complement="2625"
FT /AccII="717,727,1093,1268,1334,1423"
FT /AccII="1489,1531,1788,2006,3650,4278"
//
------------------------------------------------------------------------
I tried to parse this by using SeqIO. It didn't work.
I got an error message like below.
-----------------------------------------------------------------------
Argument "island" isn't numeric in numeric gt (>) at
/home/hy1001/bin/Bio/Location/Atomic.pm line 91, <GEN0> line 15.
Argument "island" isn't numeric in numeric gt (>) at
/home/hy1001/bin/Bio/Location/Atomic.pm line 91, <GEN0> line 15.
------------- EXCEPTION -------------
MSG: Got a sequence with no letters in - cannot guess alphabet []
STACK Bio::PrimarySeq::_guess_alphabet
/home/hy1001/bin/Bio/PrimarySeq.pm:817
STACK Bio::PrimarySeq::seq /home/hy1001/bin/Bio/PrimarySeq.pm:276
STACK Bio::PrimarySeq::new /home/hy1001/bin/Bio/PrimarySeq.pm:214
STACK Bio::Seq::new /home/hy1001/bin/Bio/Seq.pm:498
STACK Bio::Seq::RichSeq::new /home/hy1001/bin/Bio/Seq/RichSeq.pm:115
STACK Bio::Seq::SeqFactory::create
/home/hy1001/bin/Bio/Seq/SeqFactory.pm:126
STACK Bio::SeqIO::embl::next_seq /home/hy1001/bin/Bio/SeqIO/embl.pm:344
STACK toplevel extracted.pl:13
-----------------------------------------------------------------------
So I changed 'CpG island' to 'CpG_island'. Then it worked fine.
I am using perl 5.8.0 and bioperl 1.2.3 on linux.
Thank you.
- Henry.
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