[Bioperl-l] New to BioPerl
holford.5@osu.edu
holford.5@osu.edu
Wed, 27 Feb 2002 09:38:35 -0800
Does a Perl script exist for such a job or would I need to develop one?
I am in the process of learning Perl and still very wet behind the ears. I
am a C/C++ programmer and I have been asked to develop a software program
to automate a process. This process deals with parsing blast
output. Since my background in not in Biology I will not attempt to
paraphrase the request. So here it is in its entirety.
"Do you know of a program that would allow or facilitate in silico
> Northern (gene expression) analysis? What I would like to do is to take
> a specific gene sequence and blast it against the soybean EST database
> and then extract the hits below a certain E value into *categories*
> (e.g., "roots", "inoculated", etc) based on the cDNA library
> information for the particular EST in the text that accompanies the
> blast output. It could also be done based on the cDNA library #
> associated with the EST hits. This allows a quick determination of
> whether a particular gene, for instance, is root specific in its
> expression, etc. It would be extremely valuable to us in deciding what
> genes to focus on (from a gene family, for instance) for expression
> analysis with real life Northerns.
> I think that such a program would be very valuable for alot of us!"
Thanking you in advance,
Ian