[Bioperl-l] New enzymes for RestrictionEnzyme.pm
Ryan Brinkman
rbrinkman@xenongenetics.com
Mon, 12 Aug 2002 17:12:35 -0700
I just had occasion to use RestrictionEnzyme.pm, and noticed the list may be out of date (a comment says it was built on a version dated Jan 29 98").
I think the following entries can be added based on my parsing of the latest version of rebase (Aug 01 2002)
'Acc65I' => 'GGTACC 1',
'AceII' => 'GCTAGC 5',
'Asp718I' => 'GGTACC 1',
'BbeI' => 'GGCGCC 5',
'BfrBI' => 'ATGCAT 3',
'Bme1580I' => 'GKGCMC 5',
'BseBI' => 'CCWGG 2',
'BseSI' => 'GKGCMC 5',
'BsiLI' => 'CCWGG 2',
'Bsp120I' => 'GGGCCC 1',
'BssKI' => 'CCNGG 0',
'BssNAI' => 'GTATAC 3',
'Bst1107I' => 'GTATAC 3',
'BstAPI' => 'GCANNNNNTGC 7',
'BstKTI' => 'GATC 3',
'BstNI' => 'CCWGG 2',
'BstOI' => 'CCWGG 2',
'BstSCI' => 'CCNGG 0',
'Bst2UI' => 'CCWGG 2',
'BstZ17I' => 'GTATAC 3',
'BthCI' => 'GCNGC 4',
'Cfr9I' => 'CCCGGG 1',
'ChaI' => 'GATC 4',
'Csp6I' => 'GTAC 1',
'CviAII' => 'CATG 1',
'Ecl136II' => 'GAGCTC 3',
'EcoHI' => 'CCSGG 0',
'EcoICRI' => 'GAGCTC 3',
'EcoT22I' => 'ATGCAT 5',
'EgeI' => 'GGCGCC 3',
'EheI' => 'GGCGCC 3',
'EsaBC3I' => 'TCGA 2',
'FatI' => 'CATG 0',
'FmuI' => 'GGNCC 4',
'FspAI' => 'RTGCGCAY 4',
'Hin6I' => 'GCGC 1',
'HinP1I' => 'GCGC 1',
'Hpy8I' => 'GTNNAC 3',
'Hpy99I' => 'CGWCG 5',
'Hpy178III' => 'TCNNGA 2',
'Hpy188I' => 'TCNGA 3',
'Hpy188III' => 'TCNNGA 2',
'HpyCH4I' => 'CATG 3',
'HspAI' => 'GCGC 1',
'KasI' => 'GGCGCC 1',
'LpnI' => 'RGCGCY 3',
'Mph1103I' => 'ATGCAT 5',
'MroNI' => 'GCCGGC 1',
'MvaI' => 'CCWGG 2',
'NgoAIV' => 'GCCGGC 1',
'NgoMIV' => 'GCCGGC 1',
'Nli3877I' => 'CYCGRG 5',
'NsiI' => 'ATGCAT 5',
'OliI' => 'CACNNNNGTG 5',
'Ppu10I' => 'ATGCAT 1',
'PsiI' => 'TTATAA 3',
'Psp03I' => 'GGWCC 4',
'PspAI' => 'CCCGGG 1',
'PspOMI' => 'GGGCCC 1',
'PssI' => 'RGGNCCY 5',
'SciI' => 'CTCGAG 3',
'SelI' => 'CGCG 0',
'SfoI' => 'GGCGCC 3',
'Sse232I' => 'CGCCGGCG 2',
'TaiI' => 'ACGT 4',
'TauI' => 'GCSGC 4',
'TscI' => 'ACGT 4',
'UnbI' => 'GGNCC 0',
'VpaK11AI' => 'GGWCC 0',
'XmaI' => 'CCCGGG 1',
'XmaCI' => 'CCCGGG 1',
'ZraI' => 'GACGTC 3',
'Zsp2I' => 'ATGCAT 5',
Thanks for a great module,
Ryan Brinkman
Xenon Genetics Inc.
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