[Bioperl-l] avoiding temporary files

Matthew Pocock matthew_pocock@yahoo.co.uk
Wed, 17 Apr 2002 16:48:11 +0100


Does this work if $seqstring is a complete EMBL entry?

Matthew

Jason Stajich wrote:
> See the Bio::PrimarySeq or Bio::Seq perldoc man pages
> (online at docs.bioperl.org)
> 
> my $seqstring = 'CAGTGTA';
> my $seq = new Bio::Seq(-seq => $seqstring,
> 		       -display_id => 'IDNUMBER',
> 		       -desc => 'a description might go here');
> 
> 
> On Wed, 17 Apr 2002, Stephen Baird wrote:
> 
> 
>>Hi,
>>  I think this is a basic perl question but I can't figure it out and
>>since it is with my bioperl calls I'm sure one of you will know the
>>answer.  I would like to take a sequence file that is in a single variable
>>like from a textarea of a webpage and make it into a sequence object
>>without writing it to a temporary file.  It would be nice for me to do
>>this with any program that I call through perl....like blastall where the
>>query sequence does not have to be written as temporary file.
>>
>>Is this possible?
>>
>>Thanks,
>>
>>Stephen Baird
>>Molecular Genetics
>>Children's Hospital of Eastern Ontario
>>401 Smyth Rd.
>>Ottawa, Ontario
>>Canada
>>K1H 8L1
>>
>>tel: 613-738-3925
>>fax: 613-738-4833
>>
>>
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>>Bioperl-l@bioperl.org
>>http://bioperl.org/mailman/listinfo/bioperl-l
>>
> 
>