(Ewan: Sorry, i wanted to send this also to the list) I have 2 questions about the SQ Line in swissprot: does anyone know about CRC calculating used there? do i really have to add a CRC to the SQ line ?? does anyone know why the molecular weight in the "SQ" line from the "roa1.swiss" samplefile is so different from what i get by using Bio::Tools::SeqStats ?? (file says: 38715, from SeqStats: 45333) kind regards, lorenz