[EMBOSS] emma and clustalw2

Mahmut Uludag uludag at ebi.ac.uk
Thu Jan 31 16:37:37 UTC 2013


Hi Sebastien,

It looks if I set EMBOSS_CLUSTALW environment variable to point to 
clustalw2 executable, as in the following example, i don't need to 
modify the emma.acd file.

   export EMBOSS_CLUSTALW=~/esbin/clustalw-2.1/bin/clustalw2

Regards,
Mahmut

> I try to use emma (v 6.5.7.0) with clustalw2 (v 2.1) on Linux x86_64.
> My environment contains both clustalw and clustalw2, that's why
> clustalw2 binary is called clustalw2.
> Accordingly I have changed this line in the emma.acd file (before
> compilation):
>      external: "clustalw2"
> instead of
>      external: "clustalw"
>
>
> When I try to run emma, I get this error message:
>
> Multiple sequence alignment (ClustalW wrapper)
> Warning: Cannot find 'clustalw', no ACD external definition found
> Error: Failed to open filename '00011120B'
> Problem writing out EMBOSS alignment file
> Error: File 'msf::00011120B' remove failed, error:2 'No such file or
> directory'



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