[EMBOSS] specify primer3 directory?

[Petar Petrov]

Hi Paul,

which version of primer3 do you use? What operating system? How did  
you install primer3 and EMBOSS?

If it is primer3 version 2.* I think you should use eprimer32 wrapper  
instead and it expects primer3_core to be called primer32_core. To  
make primer3 aware of another location of the primer3_config  
directory: it seems that two files need to be modified before  
compiling: thal_main.c and primer3_boulder_main.c (in the subfolder  
src).

Hope this helps.

regards,

Petar


Quoting Paul Tanger <paul.tanger at colostate.edu>:

> Hi,
> I have a local install of emboss and I'm trying to get eprimer3 to
> work, but I'm getting this error:
>
> "Error: thermodynamic approach chosen, but path to thermodynamic
> parameters not specified"
>
> primer3 is installed, but not in the default location (which I think
> is /opt/primer3_config ?) .
> How do I specify where primer3 is installed?  Or is the cause of this
> error something else?
>
> googled for an answer for a while, but couldn't find one.
>
> Thanks!
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