[EMBOSS] specify primer3 directory?

Paul Tanger paul.tanger at colostate.edu
Thu Sep 13 23:03:27 UTC 2012


That worked - Thanks so much!

On Thu, Sep 13, 2012 at 4:37 PM, Drummond, Iain A.
<IDRUMMOND at partners.org> wrote:
> I ran into (I think) similar problems running primer3 with Emboss eprimer3.
>
> It turned out that the most recent version of primer3 (2.x.x) will not run
> with the current Emboss eprimer3; you have to downgrade primer3 to 1.1.4 to
> get it to work.
>
> The Emboss folks know this I think.
>
> -Iain
>
>
> -------
> Iain Drummond, Ph.D.
> Associate Professor
> Nephrology Division, Massachusetts General Hospital,
> Department of Genetics, Harvard Medical School and
> Program in Developmental and Regenerative Biology, Harvard Medical School
>
> Address for mailing:
>
> Nephrology Division, MGH
> 149 13th Street, Rm 149-8000
> Charlestown MA 02129
>
> 617 726 5647 (office)
> 617 724 9693 (lab)
> 617 726 5669 (fax)
>
> idrummon at receptor.mgh.harvard.edu
> idrummond at partners.org
>
> HTTP://danio.mgh.harvard.edu  <°){{{{-<
>
>
>
> On 9/13/12 4:24 PM, "Paul Tanger" <paul.tanger at colostate.edu> wrote:
>
>> Hi,
>> I have a local install of emboss and I'm trying to get eprimer3 to
>> work, but I'm getting this error:
>>
>> "Error: thermodynamic approach chosen, but path to thermodynamic
>> parameters not specified"
>>
>> primer3 is installed, but not in the default location (which I think
>> is /opt/primer3_config ?) .
>> How do I specify where primer3 is installed?  Or is the cause of this
>> error something else?
>>
>> googled for an answer for a while, but couldn't find one.
>>
>> Thanks!
>> _______________________________________________
>> EMBOSS mailing list
>> EMBOSS at lists.open-bio.org
>> http://lists.open-bio.org/mailman/listinfo/emboss
>>
>
>
>
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