[EMBOSS] problems with eprimer3

[Felipe Martínez-Pastor]

I replaced the eprimer3.c from EMBOSS 6.3.1, complied and, worked! Thank 
you for the help.
It is a tricky decision. By now, I suggest adding a warning in the 
eprimer3 manual page?
Regards,
Felipe

El 02/10/10 19:22, ajb at ebi.ac.uk escribió:
> Hello Felipe,
>
> Thanks for the reminder of this. Downloading the developer's version
> will not help.
>
> A version of eprimer3 that should work with primer3 2.x.x can
> be downloaded from:
>
>    http://www.ebi.ac.uk/~ajb/eprimer3/eprimer3.c
>
> if you're desperate. Note that it hasn't been tested very recently and is
> not, as yet, official. We are still debating how to deal with
> new and old versions of primer3. An odd hindrance
> is, given that it would be useful to keep the
> 1.x.x version around for compatibility reasons, what to call them.
>
> If you choose to try the above then any feedback would be welcome.
>
> ATB
>
> Alan
>
>
>
>> Dear EMBOSS developers and users,
>>
>> I am having some problems with the eprimer3 application in EMBOSS. I
>> have been using primer3 online (primer3plus, ncbi site) with good
>> results, and I just tried using it in EMBOSS. I am using MacOS 10.6.4
>> (Snow Leopard), with EMBOSS 6.3.1 installed by Fink (an UNIX porting
>> system for MacOS). The primer3 version is 2.2.3.
>>
>> One of the problems has already being described in this list, but it
>> seems to still be around. When I call eprimer3 with a sequence, the
>> output is:
>> Picks PCR primers and hybridization oligos
>> Error: Missing SEQUENCE tag
>>
>> See this thread for details, I did the same tests and the results were
>> similar; primer3_core is OK, but it seems that eprimer3 has not been
>> updated yet to successfully communicate with the primer3_core 2.x
>> versions:
>> http://www.mail-archive.com/emboss@lists.open-bio.org/msg01405.html
>>
>> I have not tried yet the developer's version of EMBOSS, should I?
>>
>> Regards,
>>
>> --
>> --------------------oOo--------------------
>> Felipe Martínez-Pastor, Ph. D.
>> ITRA-ULE (Research in Assisted Reproductive Technologies)
>> INDEGSAL (edificio Biomedicina)
>> University of León
>> Campus de Vegazana
>> 24071-León (Spain)
>> Office phone: +34 987 291 000 + 5679
>> Fax:          +34 987 291 282
>> Mobile:       +34 687 365 362
>> e-mail: felipe.martinez at unileon.es
>>           f.martinez.pastor at gmail.com
>> website: http://itra.unileon.es
>> -------------------------------------------
>>
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>
>
>