[EMBOSS] problems with eprimer3

[Felipe Martínez-Pastor]

Dear EMBOSS developers and users,

I am having some problems with the eprimer3 application in EMBOSS. I 
have been using primer3 online (primer3plus, ncbi site) with good 
results, and I just tried using it in EMBOSS. I am using MacOS 10.6.4 
(Snow Leopard), with EMBOSS 6.3.1 installed by Fink (an UNIX porting 
system for MacOS). The primer3 version is 2.2.3.

One of the problems has already being described in this list, but it 
seems to still be around. When I call eprimer3 with a sequence, the 
output is:
Picks PCR primers and hybridization oligos
Error: Missing SEQUENCE tag

See this thread for details, I did the same tests and the results were 
similar; primer3_core is OK, but it seems that eprimer3 has not been 
updated yet to successfully communicate with the primer3_core 2.x versions:
http://www.mail-archive.com/emboss@lists.open-bio.org/msg01405.html

I have not tried yet the developer's version of EMBOSS, should I?

Regards,

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--------------------oOo--------------------
Felipe Martínez-Pastor, Ph. D.
ITRA-ULE (Research in Assisted Reproductive Technologies)
INDEGSAL (edificio Biomedicina)
University of León
Campus de Vegazana
24071-León (Spain)
Office phone: +34 987 291 000 + 5679
Fax:          +34 987 291 282
Mobile:       +34 687 365 362
e-mail: felipe.martinez at unileon.es
         f.martinez.pastor at gmail.com
website: http://itra.unileon.es
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