[EMBOSS] ABI to FASTQ with seqret

[Zheng Jin Tu]

Hi Peter:

You may want to check this URL about how to 
convert quality score:

 http://maq.sourceforge.net/fastq.shtml

Thanks, TU

=======================================

On Tue, 30 Mar 2010, Peter Rice wrote:

> On 30/03/2010 14:13, Peter Rice wrote:
> 
> > Where do I look to find scores that we can use (and how do we convert
> > those to phred quality scores)?
> 
> Aha, found something. The field is called PCON (confidence values), with
> values 0-255.
> 
> There is a possibility that these could be phred scores, but I suspect they
> are whatever the basecaller has decided to write there.
> 
> http://www.appliedbiosystems.com/support/software_community/ABIF_File_Format.pdf
> 
> Peter R.
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