[EMBOSS] ABI to FASTQ with seqret
Peter Rice
pmr at ebi.ac.uk
Tue Mar 30 13:25:53 UTC 2010
On 30/03/2010 14:13, Peter Rice wrote:
> Where do I look to find scores that we can use (and how do we convert
> those to phred quality scores)?
Aha, found something. The field is called PCON (confidence values), with
values 0-255.
There is a possibility that these could be phred scores, but I suspect they
are whatever the basecaller has decided to write there.
http://www.appliedbiosystems.com/support/software_community/ABIF_File_Format.pdf
Peter R.
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