[EMBOSS] Align 12bp DNA back to genome

Aengus Stewart aengus.stewart at cancer.org.uk
Fri Feb 6 16:11:10 UTC 2009


Hi all,

This isnt an EMBOSS question per se but I thought it would go to the right audience.

After trimming a bunch of illumina reads I have sequences that are 12bp long and "we" want to find 
their genomic location

Before anyone even says "Why do you want to do that?" can I suggest

1) Treat it as a hypothetical case
2) because the scientist who asked me wants it

:-)

I have both a perl regex and fuzznuc running now for oh about a week..........

I have also attempted to build/configure/run without success

maq
soap
blat

So can anyone suggest anything else to try (probably I have missed the obvious) before the regex and 
fuzznuc finish?

This completes the Friday pm puzzler........



Cheers
Aengus

-- 
-----------------------------------------------------------------------
Aengus Stewart
Head of Bioinformatics and BioStatistics
Bioinformatics and BioStatistics               Tel: +44 (0)20 7269 3679
Cancer Research UK, Lincoln's Inn Fields, Holborn, London, WC2A 3PX, UK
-----------------------------------------------------------------------

This electronic message contains information which may be privileged and
confidential.  The information is intended to be for the use of the
individual(s) or entity named above. Be aware that any third party
disclosure, distribution, copying or use of this communication, without
prior permission, is strictly prohibited.

This communication is from Cancer Research UK. Our website is at www.cancerresearchuk.org. We are a charity registered under number 1089464 and a company limited by guarantee registered in England & Wales under number 4325234. Our registered address is 61 Lincoln's Inn Fields, London WC2A 3PX. Our central telephone number is 020 7242 0200.
 
This communication and any attachments contain information which is confidential and may also be privileged.   It is for the exclusive use of the intended recipient(s).  If you are not the intended recipient(s) please note that any form of disclosure, distribution, copying or use of this communication or the information in it or in any attachments is strictly prohibited and may be unlawful.  If you have received this communication in error, please notify the sender and delete the email and destroy any copies of it.
 
E-mail communications cannot be guaranteed to be secure or error free, as information could be intercepted, corrupted, amended, lost, destroyed, arrive late or incomplete, or contain viruses.  We do not accept liability for any such matters or their consequences.  Anyone who communicates with us by e-mail is taken to accept the risks in doing so.



More information about the EMBOSS mailing list