[EMBOSS] question about display double-stranded DNA

[Peter Rice]

Hi Jean,

> When using remap, I prefer to use the '-noreverse' flag so that the
> translation of my DNA is located closer to my DNA strand. However, using
> this flag also remove the complementary strand of my DNA in the output which
> is less convinient when design primers. Is there a way in remap to display
> double-stranded DNA but turn off the restriction sites of the complementary
> strand?

I am looking at remap changes at the moment, I will see what I can do.

> If not, is there a program in EMBOSS which can retrieve the sequence from
> database, select start/end points and display both strands? I tried seqret
> but failed.

Showseq does that.

It has a bug at present (I noticed it this week - fixed in the next release) 
that makes it show additional bases up to the end of the last line.

regards,

Peter