[EMBOSS] question about display double-stranded DNA
Jean Mao
maoj at helix.nih.gov
Thu Jan 25 13:41:55 UTC 2007
Hi,
When using remap, I prefer to use the '-noreverse' flag so that the
translation of my DNA is located closer to my DNA strand. However, using
this flag also remove the complementary strand of my DNA in the output which
is less convinient when design primers. Is there a way in remap to display
double-stranded DNA but turn off the restriction sites of the complementary
strand?
If not, is there a program in EMBOSS which can retrieve the sequence from
database, select start/end points and display both strands? I tried seqret
but failed.
Thank you in advance.
Jean Mao
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