[EMBOSS] Emboss-Digest
Sudeep Mehrotra
sum732 at mail.usask.ca
Fri Dec 7 23:01:43 UTC 2007
Hello,
I used "digest" from EMBOSS to digest protein database obtained from
NCBI REFSEQ.
Here is how I executed digest:
digest -seqall "DB_NAME" -aadata "File_name"- outfile "File_Name"
From the list I selected trypsin
For some reason, digest skipped (no fragments were generated) for this
particular protein
>gi|118430285|ref|YP_874719.1| photosystem II protein K [Agrostis
stolonifera]
MPNILSLTCICFNSVLYPTTSFFFAKLPEAYAIFNPIVDVMPVIPLFFFLLAFVWQAAVSFR
any ideas?
I should get two fragments. I don't want to see the partial digests so
that is why I never selected the option.
Thanks
Sudeep
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