[EMBOSS] restrict -limit

[Aengus Stewart]

Yeah I know, not one of my brightest days...............

Helps to look at cut position as well as motif *sigh*


Aengus



Aengus Stewart wrote:
> I seem to be having trouble with restrict not picking up -limit or am I not using it correctly?
> 
> I shouldnt be getting both BssKI and ScrFI should I or indeed both BseBI and EcoRII ???
> 
> ########################################
> # Program: restrict
> # Rundate: Wed  5 Dec 2007 13:17:08
> # Commandline: restrict
> #    -sitelen 4
> #    -enzymes all
> #    -limit
> #    -blunt
> #    -single
> #    [-sequence] rs9584819.ff
> #    -outfile /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict
> # Report_format: table
> # Report_file: /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict
> ########################################
> 
> #=======================================
> #
> # Sequence: rs9584819     from: 1   to: 101
> # HitCount: 24
> #
> # Minimum cuts per enzyme: 1
> # Maximum cuts per enzyme: 1
> # Minimum length of recognition site: 4
> # Blunt ends allowed
> # Sticky ends allowed
> # DNA is linear
> # Ambiguities allowed
> #
> #=======================================
> 
>    Start     End Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev
>       13      17 BssKI       CCNGG                12     17         .         .
>       13      17 BseBI       CCWGG                14     15         .         .
>       13      17 ScrFI       CCNGG                14     15         .         .
>       13      17 EcoRII      CCWGG                12     17         .         .
> 
> 
> 
> 
> Regards
> Aengus
> 
> 
> 


-- 
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Aengus Stewart
Head of Bioinformatics and BioStatistics
Bioinformatics and BioStatistics               Tel: +44 (0)20 7269 3679
Cancer Research UK, Lincoln's Inn Fields, Holborn, London, WC2A 3PX, UK
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