[EMBOSS] restrict -limit

[Aengus Stewart]

I seem to be having trouble with restrict not picking up -limit or am I not using it correctly?

I shouldnt be getting both BssKI and ScrFI should I or indeed both BseBI and EcoRII ???

########################################
# Program: restrict
# Rundate: Wed  5 Dec 2007 13:17:08
# Commandline: restrict
#    -sitelen 4
#    -enzymes all
#    -limit
#    -blunt
#    -single
#    [-sequence] rs9584819.ff
#    -outfile /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict
# Report_format: table
# Report_file: /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict
########################################

#=======================================
#
# Sequence: rs9584819     from: 1   to: 101
# HitCount: 24
#
# Minimum cuts per enzyme: 1
# Maximum cuts per enzyme: 1
# Minimum length of recognition site: 4
# Blunt ends allowed
# Sticky ends allowed
# DNA is linear
# Ambiguities allowed
#
#=======================================

   Start     End Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev
      13      17 BssKI       CCNGG                12     17         .         .
      13      17 BseBI       CCWGG                14     15         .         .
      13      17 ScrFI       CCNGG                14     15         .         .
      13      17 EcoRII      CCWGG                12     17         .         .




Regards
Aengus



-- 
-----------------------------------------------------------------------
Aengus Stewart
Head of Bioinformatics and BioStatistics
Bioinformatics and BioStatistics               Tel: +44 (0)20 7269 3679
Cancer Research UK, Lincoln's Inn Fields, Holborn, London, WC2A 3PX, UK
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