[EMBOSS] pepstat algorythm

[ajb at ebi.ac.uk]

>From looking at the source code:

The program uses per amino acid values for the extinction coefficient.
It gets them from the Eamino.dat data file in the EMBOSS distribution.
Extinction coefficients are based on the values of Gill and von Hippel
(1989) Anal Biochem 182 319-326.

It sums the values over the sequence and reports that. It then
calculates the 1mg/ml value by taking the above value and dividing
it by the molecular weight.

HTH

Alan

> Dear friends
>
> I would like to know how the extinction coefficients for
> proteins at 280 nm in pepstat.
>
> I read the source code and reference paper but still I
> could not understand.
>
> could somebody tell me the more informative reference
> paper or the formula of the algorithm.
>
> Thank you in advance
>
> nyachy
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