[EMBOSS] iep program for multiple protein sequences - Checked by
gbottu at ben.vub.ac.be
Fri Sep 8 07:46:32 UTC 2006
On Thu, Sep 07, 2006 at 06:27:22PM -0400, Tao Song wrote:
> I wonder can the iep program that calculates the isoelectric point of
> a protein be used
> for a protein database?
Yes, iep can take multiple proteins as input. It will create an output
file with the titration curves of the different proteins the one after
the other. It does not make one graph for all the proteins together,
which would anyway not make much biological sence.
> When asked to input protein sequence I gave 'tsw'
> instead of
> 'tsw:laci_ecoli' I got an error that said 'sequence must be protein sequence
> without BZ U X
> or *: found bad character Z'. Does iep can only take one protein sequence as
> input file?
The problem here is that iep takes as input only a protein sequence
without ambiguity symbols. The reason is without doubt that it is not
clear whether B should be handled as a Asp (negative) or a Asn (neutral),
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