[EMBOSS] Does emboss have a handy way for mutate a protein sequence

Guy Bottu gbottu at ben.vub.ac.be
Tue Mar 7 15:38:08 UTC 2006


Your solution works, as does the one proposed by David Bauer. Both are 
however rather tedious. It goes much easier with an interactive sequence 
editor. There is MSE, not a standard EMBOSS program but distributed as 
Embassadir. It runs in a VT100 terminal ; it is a little bit 
intimidating for the novice user, but you can with some practice learn to 
use it. At the BEN site we have besides MSE also installed SeaView, a 
graphical mode editor (has versions for Windows, Macintosh and X-Window). 
These editors are of course only usable if you work locally in your own 
computer or in a terminal session in a remote computer. It will not work 
if you are using a Web interface for EMBOSS ... although some Web 
interfaces might have an applet mode editor that allows to save the 
modified sequence back on the server (is there one in Jemboss ?).

	Hope this helps,
	Guy Bottu,
	Belgian EMBnet Node




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