[EMBOSS] backtranseq

Guy Bottu gbottu at ben.vub.ac.be
Fri Jul 22 09:26:38 UTC 2005


I remember that the GCG program backtranslate let the use choose between 
the most likely backtranslation (as backtranseq does) and the most 
ambiguous backtranslation. So, adding to EMBOSS a program that makes the
most ambiguous backtranslation would bring back this lost functionality.

As for the problem cases like Serine, maybe an option to make instead of a 
sequence with ambiguity symbols a regular expression that exactly matches 
the allowed codons ? The utility of this may be limited, but you could 
e.g. if you have a peptide use the backtranslation with the program dreg to
search the corresponding CDS in a piece of DNA.

	Regards,
	Guy Bottu,
	Belgian EMBnet Node




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