[EMBOSS] Antwort: needle qualifier

David.Bauer at SCHERING.DE David.Bauer at SCHERING.DE
Fri Aug 19 05:34:42 UTC 2005

I had a similar problem on a machine with some very old emboss libs
hanging around.
(in former time the parameter was named sequenceb and not bsequence)
Make sure that you have only the current libajax, libajaxg and libnucleus
in your search path for dynamic libraries.


emboss-bounces at emboss.open-bio.org schrieb am 19/08/2005 01:28:11:

> Hi there,
> I am new user and just installed the EMBOSS3.0.0 on
> UNIX.  However, when I was trying to run program
> needle, I got the following error:
> UNIX % emboss/needle
> Needleman-Wunsch global alignment.
> Input sequence: query.1
> Second sequence(s): target.1
> Gap opening penalty [10.0]: 10.0
> Gap extension penalty [0.5]: 0.5
> Output alignment [ftsh_ecoli.needle]:
> ftsh_ecoli.needle
> Died: Qualifier '-bsequence' not found
> Why is it looking for '-bsequence'?  The second
> sequence(s) have already been entered.
> Could someone help me with this?  Thanks very much!
> Emily
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