[EMBOSS] needle qualifier

emily xu emilywxu at yahoo.ca
Thu Aug 18 23:28:11 UTC 2005

Hi there,

I am new user and just installed the EMBOSS3.0.0 on
UNIX.  However, when I was trying to run program
needle, I got the following error:

UNIX % emboss/needle
Needleman-Wunsch global alignment.
Input sequence: query.1
Second sequence(s): target.1 
Gap opening penalty [10.0]: 10.0
Gap extension penalty [0.5]: 0.5
Output alignment [ftsh_ecoli.needle]:
Died: Qualifier '-bsequence' not found

Why is it looking for '-bsequence'?  The second
sequence(s) have already been entered.
Could someone help me with this?  Thanks very much!


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