[EMBOSS] maximum number of sequences for eprimer3

Iain Drummond idrummon at receptor.mgh.harvard.edu
Sat Oct 18 01:33:01 UTC 2003

I am using eprimer3 and multiple fasta formatted dinucleotide containing
sequences as input. I find that eprimer3/primer3_core will handle an input
file containing up to 23 sequences; at 24 sequences, both eprimer3 and
primer3_core ³sleep². i.e. the processes never acheive run status when I
watch what happens using top or prstat.

I don¹t know if this has something to do with limitations of my system (sun
ultra5/1GB ram/500 MB free disk space) or intrinsic to
eprimer3/primer3_core. I do know that it also happens on a Mac OS X system
(640 MB ram/35 GB free disk space).

Is there a config file that sets limits on eprimer3? (that i can change?)

Iain Drummond, Ph.D.
Assistant Professor
Department of Medicine, Harvard Medical School and
Renal Unit, Massachusetts General Hospital

Mailing address:
Renal Unit / MGH 149-8000
149 13th St. 
Charlestown, MA 02129

Tel: 617 726 5647
Fax: 617 726 5669

idrummond at partners.org
idrummon at receptor.mgh.harvard.edu

Lab Home Page:

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