[EMBOSS] Program for primer verification

Gary Williams, Tel 01223 494522 gwilliam at hgmp.mrc.ac.uk
Thu Oct 16 13:42:12 UTC 2003

Lisa Mullan wrote:
Use should eprimer3 and specify your existing primers in the
-forwardinput and -reverseinput qualifiers.

It will check that your primers are reasonable.


> -----Original Message-----
> From: owner-emboss at hgmp.mrc.ac.uk [mailto:owner-emboss at hgmp.mrc.ac.uk]On
> Behalf Of Bassi, Sebastian
> Sent: Thursday, October 16, 2003 4:05 AM
> To: emboss at embnet.org
> Subject: [EMBOSS] Program for primer verification
> I would like to know what program should I use if I want to check a pair of
> primers for self annealing, for tm, for fitness for PCR, and so on. Does any
> of the EMBOSS program will do the job? Primer3 is usefull to find good
> primers, but what I want to do is the check primers I already have selected
> by another method.
> Is there a scriptable program to accomplish this?
> Sebastian Bassi.
> Advanta Seeds. Balcarce Research Station.

Gary Williams               Tel: +44 1223 494522  Fax: +44 1223 494512
mailto:G.Williams at hgmp.mrc.ac.uk          http://www.hgmp.mrc.ac.uk/
Bioinformatics, MRC HGMP, Hinxton, Cambridge, CB10 1SB, UK

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