Hi, Group, I have one copy of EMBOSS (ver2.5.1) installed locally under cygwin/win2k. I expected "diffseq" can do the job as its name. However, I found it can not identify the different sequence at the both ends of input sequences. Only the different seq. inside can be indentified. Why? Is it the design purpose? How can I get all different sequences I need? Thank you. Chunlei