drawing trees
Jennifer Steinbachs
stein at fieldmuseum.org
Mon Aug 27 19:20:00 UTC 2001
Use your favourite alignment program...
Put your aligned sequences into PHYLIP format
Run the appropriate phylip program...
distance-based methods:
protdist (for proteins)
dnadist (for dna)
parsimony
protpars
dnapars
likelihood
dnaml or dnamlk
protml
See the phylip website
for more info (http://evolution.genetics.washington.edu/phylip.html).
If you aren't certain of the differences between the different
tree-building algorithms, you should get your hands on Nei and Kumar 2000
Molecular Evolution and Phylogenetics ISBN 0-19-513585-7. The other good
reference for phylogenetics is Hillis et al 1996 Molecular Systematics
ISBN 0-87893-282-8 Chapter 11.
-jennifer
On Mon, 27 Aug 2001, Gene Cutler wrote:
>Hello, all. I have a question about phylogenetic-type trees for
>sequences. I haven't quite figured out how to do this using
>emboss/phylip. This is how I have been doing this with gcg:
>
>perform multiple sequence alignment (generally hmmalign or clustalw)
>convert file to msf format if not already (e.g., sreformat from hmmer package)
>run gcg program distances on the msf file
>run gcg program growtree on the distances file
>end up with a postscript file
>
>How would I do this with PHYLIP instead?
>
>Thanks.
>
>
-----------------------------------
J. Steinbachs, Ph.D.
Computational Biologist
http://compbiology.org
-----------------------------------
More information about the EMBOSS
mailing list